Objective When arriving at altitude, because of the dry air, strong ultraviolet and especially the low oxygen et.al., athletes who living on the plain always exhibited kinds of stress reactions such as hyperventilation, tachycardia, dizziness, sleep-disorder, gastrointestinal disturbance and cognitive impairment in the early phase. Except these stress reactions, some studies reported the carbohydrate and lipid metabolism show significant change. Acute exposure to hypoxia could strengthen the glycolysis and suppress hepatic gluconeogenesis. The expression of some genes involved in lipid oxidation was down-regulated. However, it`s also found the increased activity of some enzymes took part in aerobic metabolism in muscle after long term acclimatization to altitude. These inconsistent conclusions make us confusion. Train load is an important factor influence the stress reactions which isn`t like as altitude travel for normal people. What would happen in the body of athletes when altitude training? We still know very little. Metabonomic give us a new tool to understand the whole map of body`s reaction to altitude training. In this pilot study, we aimed to explore the plasma profiles changes of elite rowers in the early two-weeks training at 2300m plateau using metabolic tool based on LC-MS. Methods Sixteen male elite rowers(age 25.56 ± 3.44y, height 189.06 ± 5.37cm, weight 82.81 ± 12.25kg, training years 10.31 ± 2.52y) from China National team took part in this research. This altitude training camp was organized in the preparation period of a new season. It lasted six weeks. The acute phase (AP) which was the first three days after arriving at 2300m altitude base was composed by regenerative training and low-intensity aerobic training sessions. During the next ten days (chronic phase, CP), the intensity of most sessions including three low-weight resistance training sets were low to middle aerobic. After resting half day, 5 ml venous blood was collected into heparin anticoagulant tubes and then centrifuged at 8000 rpm in 4 lasting 15 minutes to separate plasma in the 4th day and 14th morning. Plasma was stored in -80° to measure metabolic profile by LC-MS. The data was performed feature extraction and preprocessed with Compound Discoverer 2.0 software (Thermo), and then normalized and edited into two-dimensional data matrix by excel 2010 software, including Retention time(RT), Compound Molecular Weight (compMW), Observations(samples) and peak intensity. The feature data after editing were performed Multivariate Analysis (MVA) using SIMCA-P software (Umetrics AB, Umea, Sweden).
© Copyright 2018 Exercise Biochemistry Review. International Research Group on Biochemistry of Exercise. Kaikki oikeudet pidätetään. / Kääntänyt https://www.DeepL.com/Translator